Dr. Kyle M. Douglass is a researcher specializing in optical sensing, microscopy, and statistical data analysis with applications in soft matter and fundamental cell biology. Currently, he is working at the EPFL where he is developing high-throughput, super-resolution fluorescence microscopy technologies for investigating the structural biology of genes and organelles. He is financially supported by a SystemsX.ch Transition Post-doc Fellowship.
All images were taken with the help of Christian Sieben and Ambroise Lambert in the laboratory of Suliana Manley.
Telomeres are the regions located at the end of eukaryotic chromosomes and are associated with a number of important functions, from protecting chromosome ends from the DNA damage response machinery to aging and cancer development. In this work we applied to STORM to telomeres in HeLa cells to understand how and whether telomere sizes and shapes change under conditions where the DNA damage response has been triggered.
Super-resolution imaging of multiple cells by optimized flat-field epi-illumination
I designed and demonstrated an epi-illumination system for performing super-resolution imaging of multiple cells. The system is based on the fly's eye condenser and efficiently homogenizes the laser beam across the sample, which removes artifacts due to field-dependent fluorophore photophysics. The work greatly improves the throughput of localization microscopy systems.
Correction of a Depth-Dependent Lateral Distortion in 3D Super-resolution Imaging
In this paper, my co-authors and I investigated the source of a common aberration in 3D localization microscopy. We found that it is caused by aberrations in the microscope's pupil function that lack rotational symmetry and provide open-source software to correct this aberration.
Passive Optical Mapping of Structural Evolution in Complex Fluids
This paper is the result of many years of work during my PhD. We detailed the development of a low-coherence, fiber optic-based system for measuring the structural dynamics of complex polymer solutions. Furthermore, we demonstrated the capabilities of this system in an industrial triblock copolymer solution by measuring relaxation times spanning several orders of magnitude and scattering regimes from single scattering to heavy multiple scattering, which is a significant improvement over traditional dynamic light scattering methods.
Dipole–dipole interaction in random electromagnetic fields
This is a theortical paper in which my co-authors and I predict an optical binding force between two induced dipoles in a random speckle field. This counter-intuitive result is related to the Casimir effect and was experimentally demonstrated here.
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